If the Phase Initialization () study step is being used, for the initialization to work it is crucial that there are two Initial Values nodes. One of the Initial Values nodes should use Specify phase: Fluid 1 and the other Specify phase: Fluid 2. The initial fluid-fluid interface is then automatically placed on all interior boundaries between both phases. Note that when the value of Phase field variable is set to User defined, arbitrary user defined initial values have to be provided. In this case, the smooth initialization is not used, and a Time Dependent study should be used instead of Time Dependent with Phase Initialization.
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